These kits have been designed for the isolation of total RNA from a range of samples, including cells, bacteria, yeast, viruses, and bodily fluids, including plasma/serum, blood, saliva, CSF, and more ...

Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...

Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

Membraneless organelles (MLOs), also known as “biomolecular condensates,” are formed by the biological process of liquid-liquid phase separation (LLPS). MLOs are highly dynamic bodies containing ...

Membraneless organelles in cells contain high concentrations of semi-extractable RNAs, which could serve as potential biomarkers and drug targets. However, the identification and characterization of ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...

Transcriptomics represents a critical discipline in cancer research, enabling comprehensive mapping of gene expression profiles and the identification of fusion genes implicated in tumor development.

The team applied this method to mRNA/saRNA constructs from 1 to 10 kb and assessed pH ranges from 3 to 6. For all the RNA constructs, the J2 immunoblot signal—a marker for dsRNA strands—decreased with ...

In this issue of BioTechniques, we present a method for glucose detection that combines fluorescence and colorimetric techniques, a method for characterizing a single-nucleotide polymorphism in ...